Volumen: 19 # Number : 1
Publication Date : Enero - Abril Year: 2015
Study of B cells populations in HIV- infected individuals
Authors: Castro Ocampo G, Bustos A, Carelli D, Cañellas A,
Ramis E, Amuchastegui R, Arias D, Kassuha D, Larrea
Abstract: Background: HIV infection is associated to
abnormalities in lymphocyte populations, including
B cells. These disturbances lead to an increase
of specific co-infections and poor response to
several vaccines. Therefore, characterization of B
lymphocytes in HIV-infected patients is important
to understand the viral induced-immune response.
Objectives: The aim of this work was to characterize
subpopulations of B lymphocytes in HIV-positive
patients and correlate these findings with protein
electrophoresis patterns found.
Methods: 30 HIV-positive patients were included at
different stages of progression under antriretroviral
therapy. Subpopulations linfoctios B were immunotipyfied
by flow cytometry as CD24 high CD38high (B
transitional), CD27- (B virgin), CD27 + IgD + (B
memory without isotype), CD27 + IgD (B memory
isotype) and CD27 + (B total memory). Also, serum
protein electrophoresis assays were performed
using a semiautomatic analyzer and patterns were
categorized as normal, hypogammaglobulinemia,
polyclonal hypergammaglobulinemia, monoclonal
or oligoclonal bands.
Results: We found that compartment unbalances
of B lymphocytes are mainly characterized by
an increase in transitional B cell populations and
decreased memory B cells without isotype switching.
In addition, we found no correlation between the
count of CD4 + T cells and B cell counts of total
memory.
Discussion: This study shows the compartment
imbalance of B lymphocytes in adults infected with
HIV even in patients under antiretroviral treatment.
This has important implications for susceptibility to
extracellular co-infections.
Key words: HIV; lymphopenia; B cells.
Pages :
|
