Volumen: 17 # Number : 3
Publication Date : Septiembre - Diciembre Year: 2013
LABORATORIO EN HEMATOLOGÍA |
HIT Diagnosis: Anti PF4-Heparin antibodies
detection by an immoturbidimetric method
Authors: Martinuzzo ME, Barrera LH, Otaso JC
Abstract: A fast and accurate laboratory diagnosis of heparin-induced thrombocytopenia (HIT) in patients with clinical suspicion is important in order to discontinue heparin and prevent morbid thrombotic complications in patients suffering from this adverse drug reaction. In addition, the relevance of the exclusion of HIT diagnosis is given by the few alternative drugs available in our country to change the antithrombotic
therapy in these patients. The clinical pretest T4 score include: platelet nadir, time to the development of thrombocytopenia,
presence of other cause for thrombocytopenia and the development or extension of thrombosis. It is used to define the clinical probability and therefore the need for the detection of anti PF4-HEP antibodies. To investigate the presence of antibodies, ELISA tests are very sensitive, so a positive result is considered pathogenic when the absorbance
is greater than 1.000. These techniques are laborious and time consuming (at least 2 hours). Recently, rapid methods
have been developed to solve the exclusion or confirmation
of HIT diagnosis in real time. One of them is the immunoturbidimetric
method (HIT AB) that will be described in this article. HIT AB method is simple (result is obtained in 16 min), fast, takes place in a coagulometer and presents a good performance for the diagnosis of HIT.
Key words: Heparin induced thrombocytopenia,
anti PF4-Heparin antibodies.
Pages : 293-295
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