Volumen: 17 # Number : 1
Publication Date : Enero - Abril Year: 2013
Authors: Crisp RL1-3, Gammella D4, Solari L4, Rapetti MC5,
Schvartzman G3, Donato H3, 5
Abstract: Use of capillary blood. An aid for early diagnosis of hereditary spherocytosis We studied 40 children with hemolytic anemia, or with positive family history for hereditary spherocytosis,
to assess the reliability of capillary blood samples for diagnosis. Hereditary spherocytosis was diagnosed in 24 patients. Hypertonic cryohemolysis was positive in 94%, eosin-5’-maleimide flow cytometry in 90%, and flow cytometric osmotic fragility in 94% of them. Most patients with hereditary spherocytosis were positive for all performed tests. Only 6 of them presented one normal result: 2 for eosin-5’-maleimide flow cytometry, 1 for cryohemolysis test, and 3 for flow cytometric osmotic fragility. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300 μL) allows an earlier etiological diagnosis in neonates and small infants.
Key words: hereditary spherocytosis, hypertonic cryohemolysis,
flow cytometry, neonatal anemia, osmotic fragility
Pages : 8-14
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