Volumen: 16 # Number : 1
Publication Date : Enero - Abril Year: 2012
Authors: Travella Ana, Bezares Raimundo, Rodríguez Andrea, Slavutsky Irma
Abstract: Cytogenetic analysis allows the detection of structural abnormalities (SA) that cannot be observed by FISH (fluorescence in situ hybridization). Thirty four patients with diagnosis of chronic lymphocytic leukemia (CLL) and clonal SA were evaluated. Results were correlated with clinical characteristics, IGHV mutational status, and LPL and ADAM29 expression profiles. To compare clinical parameters, a control group of 78 CLL patients with normal karyotype and FISH analysis were also analyzed. RT-PCR and DNA sequencing for IGHV, and real-time PCR for gene expression were performed. Sixteen CLL patients showed new SA; chromosome 8 was the most frequently involved (7 translocations), followed by pairs 13 (6), 12 (5) and 15 (4). A similar distribution of SA in patients with IGHV mutated and unmutated was observed. Although no significant differences were found, lower ADAM29 and higher LPL expression in patients with SA compared to controls were observed. Patients with new SA showed significant differences in white blood count (p=0.019), platelet count (p=0.002), lactate dehydrogenase levels (p=0.029), treatment free survival (13 months) and overall survival (64 months) than the control group (69 and 144 months; p=0.087 and p=0,012, respectively), supporting the important prognostic value of chromosomal translocations in CLL.
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Pages : 8-19
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