Volumen: 13 # Number : 1
Publication Date : Enero - Abril Year: 2009
Authors: Elvira Gutierrez, Alina Puig, Yanelys Tirado, Antonio Bencomo, Armando Cádiz
Abstract: Obtaining of albumin from different mamalian species for inmunohematologyc use in blood banks. The albumin has a fundamental role as basic component in several biological reagents for diagnostic use. For the obtaining of this protein, hemolytic plasma from different mammalian species (bovine, equine, porcine and ovine) was subjected to a selective thermocoagulation process. In order to guarantee the necessary polymerization different glutaraldehyde and glycine concentrations were evaluated in the albumin samples. To the final products pH, protein and sodium chloride concentration, hemopigments content and polymers were determined. Some immunohematologic assays were also determined, that included: undesirable positive reaction, potentiating capability, IgG presence, substance of ABO blook groups and Lewis, and no neuraminidase activity. An average yield of 14.95 g/L was obtained, being achieved the appropriate and glycine concentration (0.06-0.08%). The physical-chemical and immunohematological evaluations carried out to the albumin samples were satisfactory, excepting the hemopigments content that although no variation was observed in the solution. In all cases, the obtained albumins could be used in the immunohematologic assays of blood banks and transfusion services.
Key words: albumin, termocoagulación, inmunohematology, polimerización
Pages : 20-26
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